TREK-1 channel blocker
PE-22-28 reconstitution calculator
PE-22-28 is commonly supplied as 5 mg, 10 mg vials. Pick a vial size and a water volume to see the concentration and what each syringe mark holds. Enter a target amount to see the draw. This page does not suggest any amount.
Work backwards: pick how many syringe units you want each draw to be, and the calculator tells you how much water gives that concentration.
Example values shown. Larger draws (10 units or more) are easier to measure accurately than very small ones.
Estimate how long a vial lasts and what a protocol costs, from your own price and schedule.
Example values shown. Replace them with your own.
Draw to
– units
– mL on a U-100 syringe
- Concentration
- – mg/mL
- Per 1 unit
- – mcg
- Draws per vial
- –
- Total in vial
- – mcg
What each mark on the syringe holds
| Units | mL | Contains |
|---|
Add
– mL
of bacteriostatic water
- Concentration
- – mg/mL
- Draws per vial
- –
Per 30 days
$–
- Cost per use
- $–
- Uses per vial
- –
- One vial lasts
- – days
- Vials per 30 days
- –
Assumes no loss to dead space in the syringe or vial. Real-world usage is usually a few percent higher.
About PE-22-28
PE-22-28 is a shortened analog of spadin, a natural peptide that blocks the TREK-1 potassium channel. TREK-1 knockout mice resist depression-like behaviour, and spadin produced fast antidepressant-like effects in mice. The shorter analogs were designed to last longer while keeping that activity.
PE-22-28 calculator questions
How much bacteriostatic water do I add to a 5 mg PE-22-28 vial?
The calculator does not prescribe a volume; the right choice depends on how large you want each draw to be. As arithmetic: 5 mg in 2 mL gives 2.5 mg/mL, so each unit on a U-100 syringe holds 25 mcg. Use the “Water to add” tab to work backwards from a draw size.
What concentration does PE-22-28 have after reconstitution?
Concentration is the vial mass divided by the water volume. For example, 5 mg in 2 mL is 2.5 mg/mL. Check the certificate of analysis for net peptide content, which is usually lower than the label.
Where can I read the research on PE-22-28?
See the PE-22-28 research profile for mechanism, study findings, safety and regulatory status, with references.